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Labeled medium was quickly removed and residual labeling on the monolayers was removed by three washes with ice-cold PBS.
Simultaneously the medium was quickly cooled to room temperature.
After 45 60 min of incubation, the medium was quickly removed and the monolayer was washed 3 times with PBS.
Culture medium was quickly aspirated from cells grown on 10 cm diameter culture dishes and cells were washed with 1 ml of ice-cold PBS.
After each incubation period, the medium was quickly discarded and the cells were immediately lysed using a protein-lysis buffer (see next subsection for buffer formulation).
Culture medium was quickly aspirated from cells grown on 6 cm culture dishes, and cells were washed with 1 ml of ice-cold PBS.
Similar(52)
The pH of the culture medium was neutralised quickly with two to three washes of serum-free DMEM medium.
Previous studies have observed that on nitrogen- or phosphate-deficient medium, vegetative growth of duckweed was quickly reduced and eventually ceased.
In the 1980 1990s, the development of nonionic contrast media (low-osmolality contrast medium, ratio: 3 iodines per molecule) was quickly adopted because of a decrease in nonkidney side effects.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com