Sentence examples for medium was quantitated from inspiring English sources

The amount of hAAT secreted into the cell culture medium was quantitated using the enzyme-linked immunoabsorbent assay (ELISA).

The amount of hAAT secreted into the medium was quantitated by ELISA using polyclonal hAAT-antibodies (HRP-coupled and uncoupled, ICN Biomedicals).

Glucose in the medium was quantitated via an Amplex Red Glucose/ Glucose Oxidase Kit (Invitrogen, Eugene, OR, USA) using a standard curve prepared with serial dilutions of RPMI 1640 (11 mmol/l glucose) into glucose-free RPMI 1640.

Glucose in the medium were quantitated using the amplex red glucose/glucose oxidase kit (Invitrogen) using a standard curve prepared with serial dilutions of RPMI medium (11 mM glucose) into glucose-free RPMI medium, as described previously [12].

The concentrations of CXCL8 (IL-8), Thymic stromal lymphopoietin (TSLP) and Interleukin 6 (IL-6) in the medium after 48 hours of medium or TLR ligand stimulation was quantitated in triplicates by enzyme-linked immunosorbent assay (ELISA; R&D, Wiesbaden, Germany).

The medium was changed once 3 days after plating and the cell number was quantitated using a Beckman Z1 Particle Counter 5 days after plating.

Briefly, cell number was quantitated by adding CellTiter-Blue reagent (Promega) to culture medium, incubating at 37 °C for 1 h and measuring fluorescent intensity (560EX/590EM).

Cell survival after heating at 44°C for 0, 15, 30, 60, 90 or 120 min was quantitated by plating cells into 25 cm flask containing the medium.

Bound [3H]9-cis-RA was quantitated by liquid scintillation counting.

Aortic intimal thickening was quantitated in H&E-stained segments.

In addition, trypan blue (TB) was quantitated by light microscopy.