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The NO amount in the culture medium was quantified by detecting its metabolism of nitrite, owing to its instability.
In the experimental study, the spatial variability in the steel weight loss along the corroded rebars in the concrete medium was quantified using X-ray and digital image processing techniques.
The concentration of drug in the release medium was quantified by HPLC method.
During the NRU assay, the Zn content of the cell medium was quantified to check the level of ZnO dissolution in the cell media.
To evaluate whether the ZnO dissolution happened or not, the Zn content of the cell medium was quantified during the procedure of the NRU assay.
In a study by Sørensen et al. [79], the behavior of CuO nanoparticles in OECD M7 medium was quantified at different concentrations over a timescale of 48 h, corresponding to an acute immobility test.
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Pro-inflammatory factors TNF-a and IL-1β in the medium were quantified using the TNF-a and IL-1β ELISA kit (Abcam, Cambridge, UK).
The levels of transferrin (TF) and prostaglandin (PG E2, PGF2α, PGD2, carbaprostacyclin (cPGI2 analog) in culture medium were quantified by ELISA.
In the table, the levels of secreted ATH35L protein in culture medium were quantified by the band intensity of ATH35L from SDS-PAGE gel using the Image J 1.50i (Schneider et al. 2012).
Levels of IFNα (PBL Biomedical Laboratories), IL-6, TNFα or IL-1β (R&D Systems) in the medium were quantified by ELISA after 24 hours of gonococcal infection.
Stx2 levels in the apical medium were quantified by ELISA.
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