Sentence examples for medium was purified from inspiring English sources

The N111R/R230G expressed in basal salts medium was purified by a three step purification procedure.

Secreted ATH35L by NSP4 in culture medium was purified and used for N-terminal peptide sequencing analysis.

To verify the cleavage of ATH35L from NSP4, secreted ATH35L in culture medium was purified and analyzed by N-terminal peptide sequencing (Additional file 1: Figure S1).

The recombinant EFE PM246 expressed in basal salts medium was purified by a three-step purification procedure with a recovery rate of about 20%.

Subsequently, the obtained culture medium was purified by a two-step centrifugation of two 15 min centrifugations at 1200 and 8000 rpm, respectively.

Sm14 from clarified culture medium was purified using a two-step procedure: anion-exchange chromatography followed by hydrophobic interaction chromatography, resulting in >95% purity with a final yield of 40% from the starting cell culture medium.

The secreted products in the culture medium were purified using a cation-exchange chromatography and a gel-filtration chromatography.

Proteins secreted into the medium were purified using Ni-chelating sepharose followed by gel filtration.

The two active β-glucosidases, one of which was mainly cell-associated while the other was present in the extracellular medium, were purified and characterized.

EPO-expressing PER.C6® cells were cultured under serum-free conditions (VPRO medium) and EPO was purified from the medium by affinity chromatography and ion-exchange chromatography.

Clones that did not yield a PCR product using direct colony-PCR, were cultured in LB medium, plasmid DNA was purified using the Wizard Plus Minipreps DNA purification system (Promega, Madison, WI), and plasmids were sequenced similarly as described above.