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WEHI-3B-conditioned medium was produced by seeding WEHI-3B cells (0.5×106 cells/mL) into the medium described above and incubating them for 3 days.
Conditioned medium was produced in transiently transfected HEK293T cells, and TOPFLASH/FOPFLASH (Upstate, Lake Placid, USA), pTOPGlow/pFOPGlow [34] TCF luciferase reporter genes or DKK1 promoter luciferase constructs WT short and TBE3,4mut short [38] were transfected to MCF7 cells as described for sHPT-1 cells [26].
Wnt3a conditioned medium was produced from mouse L-cells (ATCC) stably expressing the recombinant protein and used to treat HeLa cells.
The treated LB medium was produced by growing the pBSK-P16S-g10-mt1-rpsT bacteria clone in LB medium containing 120 μM Hg for 120 hours.
Wnt3a conditioned medium was produced using stably transfected L cells after 1 week of conditioning in medium (as previously described Sato et al, 2009) containing 10% fetal bovine serum.
Lactate, normally only detected at low levels (Green et al. 1996; Jones and Woods 1986), if at all, in C. acetobutylicum ATCC 824 fermentations in CGM medium, was produced in all three fermentations by C. acetobutylicum WUR (57 ± 7 mM).
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In air fluidization technique, autogeneous medium is produced by mixture of air and fine coal power which behaves as a separating density.
Many works using this medium were produced but few survive today because of the delicateness of the linen cloth and the solubility of the hide glue from which the binder was derived.
AFP and ALB in the extracellular medium is produced and released into extracellular fluids by cells.
In addition, we show that kynurenine in the coculture-conditioned medium is produced mainly by CAFs because IDO is not induced by COX-2 overexpression in MCF-7 cells.
During the inflammatory process which affects the endothelial barrier, an increase in the soluble forms of these molecules liberated to the extracellular medium is produced in such a way that their circulatory levels may be indicators of inflammatory processes related with cardiovascular risk [ 39].
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