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At 45 °C, about 20 mL of each medium was poured into a sterile petri dish.
Next , 2750µL of the enriched broth nutrient culture medium was poured into a test tube.
The medium was poured into sterile Petri plates and cooled at room temperature.
Next day, a second layer of the same medium was poured on the top.
(1 Between 40 and 45 °C, the medium was poured into petri dishes.
In case of the assessment for cultivated cells, the same volume of culture medium was poured in cell culture dish covered with the cells at confluence.
Similar(35)
The medium were poured into layer of a 5 mm deep.
Therefore, few drops of medium were poured into each well before inserting a ChM piece in a well.
ES cells in collagen-medium were poured either in 35-mm bacterial dishes (1.5 ml per well) or in 12-well bacterial microplaque (1 ml per well) at a concentration of 2,000 cells/well, leading to the obtention of a 1.5 mm-thick or a 2.4 mm-thick gel, respectively.
Agar medium (20 ml) was poured into each Petri plate and plates were swabbed with 100 µl inocula of the test microorganisms and kept for 15 min for adsorption.
Sterile medium (50 ml) was poured onto a plate (150 mm diameter, FALCON 351058, Becton Dickinson Labware, NJ).
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