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Briefly, 1 ml of J774 cells (105 cells/ml) in culture medium was plated into 24 well plates and incubated for 24 hours at 37°C and 5% CO2 before start of the assay.
Briefly, a base layer of 0.6% agar in complete medium was plated in six-well plates and allowed to solidify (0.5 µg/ml Puromycin was added to wells containing shERβ transfected cells, while 250 µg/ml G418 was added to ERβ transfected cells).
After 1 h, an aliquot of the medium was plated to confirm the elimination of extracellular bacteria, and the gentamycin-containing medium was washed from the monolayer.
On the fourth day, one loopful of the cultured medium was plated both on Salmonella-Shigella agar (Difco) and Leifson agar (Merck).
In Oslo, 100 μL of each CSF sample in trans-isolate medium was plated onto chocolate agar and chocolate agar containing 7.5 mg/L colimycin, 0.5 mg/L linocmycin, 1.0 mg/L amphotericin B, and 5.0 mg/L trimethoprim.
A total of 10 µL of thawed, inoculated STGG medium was plated onto gentamicin blood agar (GBA) plates for selective isolation of Streptococcus pneumoniae and incubated for 18 24 hours at 35°C in 5% CO2.
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104 cells/well in 90 μL of complete culture medium were plated in a 96-well plate and incubated for 24 h at 37 °C and 5% CO2, before adding 10 μL of either SLN or sorafenib in the culture medium.
Two thousand cells in 200 µl medium were plated into each well of 96-well plates and incubated overnight to allow cell adherence.
The cultured glioma cells and co-cultures with hUCBSC (100 μL medium) were plated in 96-well microtiter plates at a final concentration of 0.5×105 cells/well.
Subsequently wells were washed three times with PBS and 2×105 CD4 positive mouse T cells in 200 μl culture medium were plated on each well.
Lymph node cell suspensions in complete medium were plated at a concentration of 2×105 cells per well in 96-well plates in the presence of Ab2-β (5, 10 and 15 µg/mL) or 10 µg of gp43.
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CEO of Professional Science Editing for Scientists @ prosciediting.com