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For this purpose, a patch of colony on agar medium was picked to culture in liquid medium for three days; different stress conditions were then applied by adding sorbitol or NaCl, or by transfer to liquid medium lacking glucose or nitrate, and the cells were cultured for an additional eight hours.
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The colony grown on SD selection medium were picked up and then confirmed by the PCR method using the primer pair up100-F and hstg2-R whether the expression plasmid was inserted into the genomic DNA (Table 1).
From the inoculated solid LB medium, one bacterial colony was picked and cultivated in the 10 mL of liquid LB medium that contained 50 ug/mL of Kanamycin and Ampicillin.
In each of the segment, a particular size of the sample constituent - large, medium and small particles - was picked round to give almost equal representation of the segment, and the sample at large.
Then mycelia dishes of approximately 4 mm diameter were cut from culture medium, and one of them was picked up with a sterilized inoculation needle and inoculated in the center of PDA plate aseptically.
A single white colony was picked and transferred to liquid medium containing 10 mM acetate and perchlorate.
One single colony was picked and transferred to the liquid medium mentioned above for further culturing.
A single colony was picked and streaked into new Zobell 2216E medium.
A single colony was picked up, homogenized, and cultured in liquid B medium [ 28].
After 7 days of growth, a single colony was picked and used to inoculate a Bold's medium liquid culture.
A single colony was picked and used to inoculate 5 mL of LB-ampicillin medium.
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