Sentence examples for medium was passed from inspiring English sources
The concentrated conditioned medium was passed twice through a diethylaminoethyl (DEAE) column at 4 °C, washed extensively in a HEPES buffered solution (pH 8.0) containing 250 mM NaCl, 0.5 mM phenylmethylsulfonyl fluoride (PMSF), 0.5 mM benzamadine, and 0.2%% (w/v) sodium azide.
During this three-month period of incubation, the medium was passed through 0.22 µm Millipore filter if contamination was suspected.
For fractionation experiments, concentrated medium was passed over a heparin-agarose column and the flow-through and eluted fractions were collected and reconcentrated using the same ultrafiltration system.
A culture medium was passed through a 0.45-μm-pore-size filter (Durapore Membrane, Millipore).
Crude expression medium was passed over a Ni2+-NTA column using gravity.
Cells and debris were removed by centrifugation, and the medium was passed through a filter (pore size 0.45 μm).
To prepare inocula, fungal cultures grown for 5 days in the liquid Czapek-Dox medium were passed through several layers of cheesecloth (to remove mycelia), and the conidial concentration was adjusted to approximately 1×107 conidia per ml.
Before using the samples for HPLC analysis, the cell lysate and medium were passed through Sep-Pak C18 cartridge columns (Waters Corp., Milford, MA) to collect the 125I-labeled Ang-(1 12) metabolic products.
A soluble form of LERP, expressed in Spodoptera frugiperda (Sf9) cells, was immobilized on an affinity resin and S2 cell lysates or medium were passed over the column as a source of Drosophila lysosomal enzymes.
MONO-MAC-6 cells (2 × 106 cells/experiment) suspended in 5 mL of medium were passed through (sucked-and-poured) twice by the LeukoCatch, and then were divided into two tubes.
The biological sample, such as plasma or cell culture medium, is passed through filters with an appropriate pore size (0.1 1 μm) that are made of materials that do not bind particles nonspecifically [ 92– 92].
