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In the first step, the growth medium was optimized by using screening designs for finding the optimal carbon and nitrogen source.
Therefore, the medium was optimized.
In this study, the medium was optimized using different concentrations of K2HPO4 and MgSO4·7H2O.
Frequency of the addition of the oxidants in aqueous medium was optimized.
The reaction medium was optimized to simultaneously allow specific enrichment of Salmonella and maximize the staining of the target pathogen.
As such, an efficient single step affinity process to purify recombinant proteins from serum-containing medium was optimized.
Similar(47)
The culture and nutritional parameters associated with unoptimized LM10 medium were optimized using statistical approach to maximize the recovery and enumeration of endolithic bacteria.
The concentration of NaNO3, MgSO4·7H2O and proteose, in Chlorella protothecoides medium were optimized for algal biomass and lipid production by using response surface methodology with Box-Behnken design.
The details of culture conditions including inoculation ages, inoculums volume, and initial pH of fermentation medium were optimized in our previous work (data not shown and detailed data in Additional file 1: Figure S1).
Carbon and nitrogen constituents of the culture medium were optimized using a 22 factorial design with central point followed by a multiple response optimization coupled to the desirability function approach.
Thus, the number of control HeLa p0 cells as compared to HeLa p0 cells containing internalized HeLa mitochondria may have been disproportionally overrepresented due to the medium being optimized specifically for their growth.
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