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To simplify interpretation, exogenous epidermal growth factor (EGF), which is normally present at 1 ng/ml in organotypic culture growth medium, was omitted from the medium at the time of treatment with 4-HT in all experiments.
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Here, washing steps and preincubation steps with serum-free medium were omitted.
The vitamin solution and cysteine used previously in BA medium were omitted, and instead the medium was supplemented with 2 g/L yeast extract and the appropriate monosaccharide substrate at a concentration of 10 g/L.
If an examination has failed because the patient has moved or contrast medium administration was omitted, it is not enough to state this in the report.
For the cell wall-deficient arginine-requiring strain 330, arginine 100 μg mL-1 was added to the TAP medium but was omitted from TAP-N.
EBs were formed by the forced aggregation of 2,000 (HES3) or 3,500 (MEL1) hESCs in APEL (the protein-free hybridoma medium component was omitted from this formulation) containing 10 ng/ml bone morphogenetic protein 4 (BMP4) and 150 200 ng/ml activin A (batch dependent) in low-attachment 96-well plates.
After 24 hours, the medium was replaced, FBS was omitted and the different phenolic acids (10-8 M) were introduced.
In this report, we show that XprG has a wider role as a global regulator of the carbon nutrient stress response and is involved in triggering autolysis, a form of fungal programmed cell death induced by starvation. A. nidulans was cultured at 37°C in Aspergillus complete or minimal medium except that glucose was omitted from media that contained other carbon sources.
The gene OPN1MW (medium-wave/green cone opsin) was omitted from further analysis since its orthologous sequences could not be reliably retrieved for all species; aside from the human and orangutan, only one member of the tangential gene sequence consisting of OPN1LW and one or more copies of OPN1MW is present in each of the other Old World monkey genome assemblies.
For growth in protein-free medium to evaluate the activities of specific growth factors, FBS was omitted from the medium.
To prepare the medium without nitrogen (TAP-N), ammonium (NH4Cl) was omitted from the regular TAP medium described above.
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