Sentence examples for medium was next from inspiring English sources

The antibody treated medium was next added to human middle ear epithelial cells transfected with the β-defesnisn 2 promoter construct.

In Group D and Group E with BPA-BNCT, cells were incubated for 24 h in medium with BPA and washed three times with phosphate-buffered saline (PBS); the medium was next replaced with boron-free medium; cells were exposed to the XAPR with irradiation doses of 4 Gy and 8 Gy, respectively.

Medium was changed the next day and new media collected the following day.

Medium was replaced the next day with medium that had been exposed to the rhBMP-2-releasing PlnD1-conjugated PCL constructs (or unmodified PCL scaffolds) from the previous time-point, and cells were incubated with this medium for 3 days.

The supernatants were removed, and fresh medium was added the next day.

For serum deprivation studies, the medium was replaced the next day (Day 0) with medium containing 0.1% FBS.

The medium was changed the next day either to a fresh control or to osteogenic medium, containing 50 µg/ml L-ascorbic acid-2 phosphate, 10 mM glycerol 2-phospate disodium salt and 10−8M dexamethasone (all from Sigma, IL).

The medium was changed the next day.

The medium was replaced the next day.

The medium was changed the next day (day 1).

Fifty percent medium was added the next day, and medium was changed every other day thereafter.