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PGE2 in the culture medium was measured by ELISA.
The amount of endostatin released by the cells into the medium was measured using an ELISA.
The activity of MMP secreted into culture medium was measured by zymography using gelatin and collagen as substrates, as described previously52.
The attenuation of 59.5 keV gamma-rays from the Am-241 through the hydrogen medium was measured by using an HPGe detector.
The content of CyA in the medium was measured by HPLC method as described above.
In this study, the pH of the reaction medium was measured at the 1 min interval.
The content of propofol in the medium was measured by HPLC method as described above.
The content of remaining CyA in the medium was measured by HPLC method as described above.
The concentration of NO in the cultured medium was measured indirectly by analysis of nitrite levels using the Griess reaction.
The amount of TNF-α released from macrophages into the culture medium was measured by ELISA (Additional file 4).
TC concentration reduction in the medium was measured using UV/Vis spectroscopy at λ max = 357 nm.
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