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The PW-GPY medium was loaded into Erlenmeyer flasks and heated to 100°C rapidly.
As an experimental control, Bacto Casitone medium was loaded in all assays (25 μL).
Briefly, 40 ul of medium was loaded onto 7.5% polyacrylamide gel containing 1 mg/ml gelatin.
For each fermentation sample, 100 mL of fermentation medium was loaded into a 500-mL Erlenmeyer flask and incubated in a constant-temperature vibrator shaking at 120 rpm.
The collected medium was loaded into ultracentrifuge tubes and spun in a SW28 rotor for 2 hours at 19400 rpm in a L8-80M ultracentrifuge (Beckman).
An aliquot of 10 μL culture medium was loaded onto a SDS-PAGE gel under non-reducing conditions using an 8% polyacrylamide gel containing 0.1 mg/mL gelatin.
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The sample is held together with a few micron-sized chips of ruby, which are used as pressure gauge, and a pressure transmitting medium is loaded into the hole in the gasket.
2.0×105 T2 cells in serum-free medium were loaded in the upper chamber.
Twenty microliter of the culture medium were loaded on 10% Tris-Glycine gels containing 0.1% gelatin.
An equal number of cells (2.5 × 10/ml) in 0.5 ml medium were loaded into the top chamber of each well.
Briefly, 1 × 10 endothelial cells in 250 μl endothelial cell culture medium were loaded into each insert.
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