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The temperature of the growth medium was kept constant at 25° in all the experiments.
A specified amount of culture medium was kept in the same condition as a negative control.
The pH of the medium was kept constant during the experiments, showing that molasses presents a buffering effect.
The reaction medium was kept under stirring for 2 days in an argon atmosphere at room temperature.
The concentration of DMSO in the medium was kept 0.1% so that it has no effect on the cell proliferation.
The medium was kept at 4 °C overnight and then centrifuged at 12,000 × g for 30 min.
Similar(34)
The optimum combination of inorganic salts for carotenoids yield was evaluated with the non-linear optimization algorithm as follows: MgSO4 0.12 g/L, Na2HPO4 2.05 g/L, FeSO4 0.03 g/L, Na2CO3 2.22 g/L, and the other components of fermentative medium were kept the same as basal medium (citric acid 8.1 g/L, NH4Cl 3.5 g/L, corn syrup 1.8 g/L).
Dishes with M16 medium were kept at 37°C in a 5% CO2/air mixture, and dishes with M2 medium were kept at 37°C in air.
If vancomycin-containing BCDA medium is kept in cool, dark location, allow the medium to come to room temperature.
3) DTT in plate survival assays is not ideal as it oxidizes over time unless the medium is kept at a low pH.
The samples in 2 ml cryovials containing virus transport medium were kept at 4°C and transported twice per week to the Pasteur Centre of Cameroon (PCC) for analysis.
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