The sterilized medium was inoculated with two 5 mm mycelial plugs, and a non-inoculated medium was used as the control.
Briefly, a starter culture of 5 mL of Luria-Bertani (LB) medium was inoculated with a single colony and grown until OD600 reaches 0.6.
Three liters of TSB medium was inoculated with 5% (v/v) seed culture supplemented with thiostrepton and incubated at 28 °C and 250 rpm for 2 days.
The medium was inoculated simultaneously with a laboratory-grown bacterial culture and the phage preparation (diluted to a concentration of ~107 bacteria and 106 phage particles per ml), and incubated at 37 °C with shaking.
The medium was inoculated simultaneously with a laboratory-grown bacterial culture and the phage preparation (diluted to an initial concentration of approximately 107 bacteria and 106 phage particles per ml), and was incubated at 37 °C with shaking.
LB medium was inoculated simultaneously with a laboratory-grown bacterial culture and the phage preparation (diluted to a concentration of ~107 bacteria and 106 phage particles per ml), and incubated at 37 °C with shaking.
After sterilization, the fermentation medium was inoculated with 5% (v/v) seed medium.
The medium was inoculated with bacterial strain and then incubated at 45 °C for 72 h.
The medium was inoculated directly with 1*106 conidia, a pre-culture was not used.
Then, the same medium was inoculated with 2% seed culture, and grown until OD600 reached 0.6.
The production medium was inoculated with 0.4 mL of a standardized bacterial inoculum.
