Sentence examples for medium was immediately from inspiring English sources
After incubation, medium was immediately removed and cells were washed with ultrapure water for five times.
After incubation, the medium was immediately removed and cells were washed with ultrapure water for five times.
The remaining medium was immediately replaced with fresh medium and cytotoxicity was determined by MTT assay (see Cell vitality).
Subsequently, the cells were nucleofected by using the U30 program from the nucleofection device and 500 µl of pre-warmed culture medium was immediately added to the cells.
The effect of trehalose inclusion in the freezing medium was immediately evident from the morphological appearance of the dry cells observed with Scanning Electron Microscope (SEM).
50 ul of complete culture medium was immediately added to pulsed samples and the total solution was transferred to an Eppendorf tube.
Samples of the incubation medium were immediately frozen for insulin analysis.
The cover slips mounted on glass slides with 15 µl Mowiol mounting medium were immediately observed via a Leica AOBS TCS SP5 inverted laser scanning confocal microscope.
The release medium collected was immediately replaced by 1 mL fresh FCS or HS, so that the volume was kept at 6 mL until the end of the study.
Medium containing serum was immediately added and the cells plated on 90 mm dishes at 5 × 10 cells per dish before selection in hygromycin (150 μg μl−1) for 10 14 days.
She has improved her illustration and said the medium was more immediately gratifying than sculpting.
