Sentence examples for medium was further from inspiring English sources

Acidic treatment was performed one hour later by replacing the culture medium with DMEM acidified with citric acid (Sigma-Aldrich) at pH 5.0 for 15 min. Medium was further aspirated and replaced by complete medium.

If phages were to be added, the medium was further supplemented with 10 mM CaCl2.

On day 9, this medium was further supplemented with 10 ng mL−1 EGF (Peprotech) and 10 ng mL−1 FGF2 to help maintain the cells at the pancreatic progenitor stage.

This medium was further supplemented with sucrose (1%) and ammonium citrate (50 mg/l) on day 10 and sucrose (1%) and phenylalanine (0.1 mM) on day 20 (i.e., biomass growth medium).

Initial experiments showed that the maximum production of URAK occurred at 12th h at pH 9. The efficacy of the medium was further studied in a fermentor as batch fermentation with the same medium optimized by RSM and the yield was comparable with that of the shake flask culture.

This medium was further evaluated with 116 soil samples.

The effect of different f numbers on the focusing performance in the attenuative medium is further investigated.

Moreover, the electrocatalytic performance of GC-WC for the oxygen reduction reaction (ORR) in alkaline medium is further investigated by cyclic voltammetry (CV), rotating disk electrode (RDE) and chronoamperometry test with or without 3 M methanol.

To overcome the limitation in online viscosity measurements, Darcy's law describing the flow of a fluid through a porous medium is further introduced to link the electrolyte viscosity to the pressure drop across a porous medium where the electrolyte solutions flow through.

Isolated strains presenting clear colonial morphology on LB agar culture medium were further purified and incubated in LB culture media of 200 mL at 30 °C.

Also, analysis of gene essentiality of Y. lipolytica cells growth in minimal growth (MG) medium or yeast extract (YE) medium was conducted, and the essential genes predicted based on four Y. lipolytica models in YE medium were further verified by database of essential genes (DEG).