Sentence examples for medium was fixed from inspiring English sources

Final electron donor concentration in the NBAF medium was fixed to 25 mM for both ethanol and pyruvate.

We assumed that the parameters for the 1D host medium were fixed and known.

Cells that had previously been washed off the medium were fixed with 4% paraformaldehyde solution for 15 min. In order to permeabilize the cells, 0.1% Triton X-100 (Sigma) detergent was added to the prefixed cells for 15 min. Then, the cells were rinsed twice with phosphate-buffered saline (PBS).

Cell monolayers cultured in adipogenic medium were fixed in 10% NBFS for 10 mins.

Jurkat cells in culture medium were fixed 1∶1 (v/v) with 4% paraformaldehyde+0.4% glutaraldehyde pH 7.4 in 0.2 M PHEM buffer (60 mM PIPES, 25 mM HEPES, 2 mM MgCl2, 10 mM EGTA) for 30 min at room temperature.

Coverslips containing cortical neurons subjected to either control Neurobasal medium or GD medium were fixed in 4% buffered paraformaldehyde in PBS.

Fifty one of these SLESs in the minimal and two in the YP medium were fixed by using the global modifications found for GNGs.

Cells treated with osteocyte or adipocyte differentiation medium were fixed in the same manner on days 3, 7 and 14 of treatment.

Cells treated with regular MSC medium were fixed in 3%formaldehyde/2%% sucrose for 10 minutes at room temperature when approximately 80% confluent.

The nitrogen and sea salt concentrations in each medium were fixed at 4.4 mM and 2%, respectively, to normalize the effects of the medium composition on cell growth and lipid accumulation.

MPCs cultured for 3 weeks in osteogenic medium were fixed with 60% isopropyl alcohol and stained for 3 minutes with 2% (wt/vol) Alizarin red S (Rowley Biochemical Inc., Danvers, MA, USA) at pH 4.2 to detect mineralization.