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Addition of NPs into about 60 °C autoclaved agar medium was finally used as an alternative procedure in which no NP aggregation was detected and sterilizing process was still effective.
A 24 h incubation in the presence of 150 nM PMA followed by 24 h in control medium was finally selected as differentiation protocol.
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That's still largely true, though it's at least changing for the better, as the medium is finally wrestled from the grasp of network broadcasters and cable companies.Attendees at last week's Consumer Electronics Show (CES) in Las Vegas saw many technology trends converging on the television set.
To A549 and HEK293 cells, additional serum-free medium (100 µl) was finally added, resulting in a total volume of 200 µl in each well, that had been seeded with 10,000 cells 16 h prior to the experiment (see above).
For inoculating main cultures, fresh precultures (grown to an OTR of ca. 0.05 mol/L/h) were centrifuged, washed in 5 mL fresh medium, centrifuged again and the pellet was finally resuspended in 5 mL medium.
The resulting pellet was finally re-suspended in culture medium.
After further washing, the cell stock was finally resuspended in 1 ml of GC culture medium.
From the viewpoint of energy efficiency and adjustability, the W-shaped underground heat exchanger with moderate medium flow rate is finally adopted for the energy pile system.
After the pellet was resuspended in fresh CM medium, the cells were finally cultivated 14 days at 27°C under permanent illumination (16.4 W/m).
After 1 h of phagocytosis, the medium was replaced and then finally collected for exosome isolation after 48 h.
Finally, the medium was removed and the precipitated formazan crystals were dissolved in optical grade DMSO.
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