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Then DHP CCBs prepared in perfusion medium was fed to the kidney for 30 min.
It is worth noting that microbial contamination was not observed even when non-sterilized medium was fed.
Sterile or non-sterile fermentation medium was fed using two peristaltic pumps (Cole-Parmer Company, Barrington, IL, USA) accurately synchronized to feed fresh medium and to extract fermented medium.
The medium was fed into the fermentor at a dilution rate (D) of 0.17 h-1.
The feed medium was fed exponentially into the fermenter using a variable speed peristaltic pump.
The medium was fed through a sample point located 0.07 m above the graphite granules.
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Clostridium thermocellum DSM 1313 was grown at 55 °C in duplicate 1 L (total vessel capacity 1.3 L) chemostat cultures using water-jacketed BioFlo110 bioreactors (New Brunswick Scientific, Edison, NJ, USA) with 1.1 g/L cellobiose as the carbon source in MTC medium, which was fed at a dilution rate of 0.1 h−1.
A defined medium containing glucose was fed at a flow rate of 1.6 mL/min (0.096 L/h) into the capped reactor, producing a hydraulic retention time of 2.1 min.
The amount of accumulated ethanol on the mixed-substrate was similar to the amount of ethanol accumulated in the medium when the chemostat was fed with ethanol alone before the washout occurred The biomass yield on substrate was substantially decreased when the dilution rate was increased on all substrates used in this study.
As shown in Figure 3, after 13 h of batch culture, concentrated P2 medium (500 g/L glucose) was fed into the reactor via a peristaltic pump.
When glucose was decreased to <10.0 g/L, the concentrated medium containing ~400.0 g/L glucose was fed into the bioreactor till the fermentation ended.
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