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Hexadecane present in the culture medium was extracted using a liquid liquid extraction procedure.
Additionally, in two schemes with flue bypass when the medium was extracted, the thermal efficiency of boiler could be improved as well.
Lipids were extracted from the yeast cells employing the chloroform methanol 2 1 method adopted by Chen and Chen (2014). 1 ml of the culture medium was extracted from the fermentation flask.
The resultant medium was extracted with 8 ml Chloroform/Methanol (2∶1), and the aqueous phase was collected and used for radioactivity measurement in a liquid scintillation counter.
The intraliposomal medium was extracted by treating LP-49 liposomes with 1% Triton X-100 followed by centrifugation at 50,000 rpm in a total volume of 100 µl of reconstitution buffer.
The medium was extracted with chloroform and centrifuged.
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Independent results from two laboratories suggest that including the swab in the lysis step does not lead to higher influenza A virus recovery, and that recovery is less variable when only the swab storage medium is extracted.
First, exosomes from the conditioned medium were extracted by ExtraPEG or UC method.
Then, proteins from the conditioned medium were extracted based on acetone precipitation.
The metabolites in the filtered medium were extracted by using iced 50% methanol and were subsequently dried by a speedvac.
Lipids in the cells and the basal medium were extracted with chloroform/methanol (2 : 1, vol/vol), and the chloroform extract was washed with methanol/water (1 : 1, vol/vol).
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