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As shown in the Figure 4B, the xanthine of the medium was exhausted much earlier under high DO level than low DO level.
Hence, the evolved strain was apparently unable to utilize the ethanol produced after the glucose in the medium was exhausted.
After the glycerol in the medium was exhausted, the fed-batch phase (phase II) was initiated by feeding limited glycerol to allow further cell growth.
As shown in Fig. 4, the glycerol in the initial medium was exhausted after 12 h of fermentation, and a glycerol solution (50 %, w/v) was then fed at a rate of 7.5 g/h for 6.5 h.
When i = cysteine is considered as substrate, the assumption was made that cysteine could be synthesized by the cells at a fixed rate r cys when additional sulfur (i.e. (NH4 2SO4) was present in the medium (as in K4, K5, K7, K8) and cysteine provided in the medium was exhausted after approx. 40 h of cultivation.
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Here, the induction is triggered automatically for T7 E. coli expression systems by lactose when the other carbon source (glucose) in the medium is exhausted, thus circumventing the effect of induction at different growth phases.
After mannose was exhausted from the medium, the relatively low amount of galactose (4 g.L−1) was co-consumed together with xylose (data not shown).
When glucose was exhausted in the medium, ppGpp accumulated in the wild type and ΔrelA spoT+ strains, but not in ΔrelA ΔspoT strains (Figure 2B).
An increase in the pressure of oxygen partially speeded up the dye degradation and increased the dye degradation rate until the oxygen was exhausted in the reaction medium.
She was exhausted.
O'Sullivan was exhausted.
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CEO of Professional Science Editing for Scientists @ prosciediting.com