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To standardize the kairomone concentration for all kairomone treatments, medium was enriched with Notonecta kairomones and pooled prior to the daily medium change.
The fatty acid profile indicated differences when the medium was enriched with Na2CO3 and cultured in high light intensity, resulting in an increase in unsaturated fatty acids, particularly linoleic acid, which accounts for 40% of the fatty acids in Synechococcus sp. PCC7942.
The culture medium was enriched with fertilizers whose composition was presented in Table 1.
The medium was enriched for plating with 2% of fetal calf serum (v/v).
The medium was enriched with 5 ml of a trace element solution containing per litre 0.29 g ZnSO4·7H2O, 0.19 g CaCl2·2H2O, 0.25 g CUSO4·5H2O, 0.17 g MnSO4·7H2O.
The rate of alamar blue dye reduction was determined by the activity of enzyme dehydrogenase; first broth nutrient culture medium was enriched with KH2PO4 (3.28 g/L), K2HPO4 (5.28 g/L), sodium acetate (0.4 g/L) and glucose (0.4 g/L).
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In addition, MSpresso-predicted proteins from yeast grown in minimal medium are enriched for small molecule metabolism (P-value < 0.001), which is expected for growth in minimal medium.
In addition, genes that are not expressed in nutrient-rich medium are enriched in non-conserved regions (Lines D in Figure 3).
The media and cells were harvested 48 h post-treatment, the proteins in the medium were enriched as above, pelleted by centrifugation, and dissolved in 100 μl SDS-PAGE sample buffer.
The control medium (N-replete) was enriched with vitamins and trace-metals (F/2 medium; Guillard & Ryther 1962).
Biosurfactant production medium without trace elements was enriched with a broad range of Fe NPs (0.01 to 1000 mg/L) and the growth rate of actinobacterium during the fermentation process was estimated based on OD at 600 nm (UV vis spectrophotometer AU-2701).
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