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The ionomycin-induced appearance of Met-NTF in the conditioned medium was efficiently inhibited by the metalloprotease inhibitor TAPI (TNF- α processing inhibitor), but not by the calpain inhibitor calpeptin.
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In this report, we were interested in studying the PBMC transcriptome at the time when cytokines released in the medium are efficiently measured.
Importantly, most of the α-amylase activity was detected in the culture medium, confirming that the protein was efficiently targeted to the secretory pathway of the yeast cells (Fig. 1), which greatly facilitated the subsequent purification step.
HPLC analysis demonstrated that TNT was efficiently removed from the medium during yeast growth (Figure 1A).
Wild-type (wt) matrilin-3 was efficiently secreted into conditioned medium, whereas mutant matrilin-3 was retained and accumulated within the cell.
This pRc-CMV/FLAG-rJerd construct was transiently expressed in HEK-293 cells and was efficiently secreted into the culture medium.
After induction with nisin, the His6-TEV-FtsL fusion protein was efficiently secreted into the culture medium of L. lactis (Fig. 3).
The d2-propargyl tosylate 6 was efficiently obtained (40% in two reaction steps) from alcohol 5 by reaction with tosyl chloride in basic medium at room temperature.
The model anticancer drug docetaxel was efficiently encapsulated (around 75%) and retained within the nanocapsule's structure upon dilution in a simulated physiological medium.
(His partner, Maria Riccetto, was efficiently bland).
One of the hirudin variants HV3 was efficiently expressed in Escherichia coli using the l-asparaginase II signal sequence and the product was secreted into the culture medium.
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