Sentence examples for medium was diluted from inspiring English sources

After incubation for 24 h, 1 mL medium was diluted, and 0.1 mL each of 107 dilutions was plated on nutrient agar and incubated for 24 30 °C in dark, and colonies were directly counted and expressed as CFU/mL.

Briefly, the medium was diluted by Milli-Q water×200; 25 µl of the diluted medium and 100 µl osteocalcin antiserum were placed in 96 well EIA plates and incubated at 4°C for 18 24 hours.

The collected medium was diluted to 5 ml with PBS and further diluted by 15-fold with ethanol.

For the growth assay in liquid culture, each overnight culture cultivated in a test tube containing 3 mL of M9 minimal medium was diluted 1 100 with fresh M9 minimal medium to obtain a total volume of 3 mL, before growing overnight in test tubes with agitation.

A 50 µL aliquot of the cell fraction (suspended in medium) was diluted with 100 µL medium and 50 µL trypan blue (0.4% in H2O).

The medium was diluted 1 10 with the specimen diluent, and the assay was carried out according to the manufacturer's directions.

Overnight E. coli cultures, grown in LB medium, were diluted 100-fold in LB medium and shake-incubated at 37 °C, 180 rpm, to a OD600nm = 0.6 cell density.

Overnight grown yeast cells in YPGal medium were diluted to OD600 = 0.3 and grown for 4 h at 30°C.

For ether treatment, cells in culture medium were diluted 1∶2 with ethyl ether, mixed, and after 10 minutes, samples were removed, diluted, and titered.

Exponentially growing cells in PAB medium were diluted back into fresh PAB medium with or without 1% xylose to inducce overexpression of GFP-MinC/MinD.

Total cell lysates and culture medium were diluted 1∶50, and hPGRN concentrations were detected with an ELISA kit (Alexis Biochemicals) according to the manufacturer's instructions.