Sentence examples for medium was decanted from inspiring English sources

After culturing, the medium was decanted and wells were gently rinsed with phosphate-buffered saline (PBS, pH 7.4) (Medicago AB, Uppsala, Sweden).

After 5 days the medium was decanted and the fungal pellets were resuspended in 100 mL of fresh Kirk's medium containing 0.12 mm ammonia tartrate (low-N medium) and 83 μM (50 ppm) amaranth.

For PGE2 measurements, medium was decanted and the monolayers covered with phosphate-buffered saline in the presence of IL-1β for the final 30 min of the incubation.

After absorption for an hour at 37°C, the medium was decanted to remove unbound VSV, followed by addition of 100 µl/well of culture medium containing 1% methylcellulose.

Cell medium containing 10% BCS was added to cells to inactivate trypsin and the cells pelleted at 400 x g for 2 min. The medium was decanted and the cell pellet resuspended in freezing medium consisting of cell media containing 20% BCS and 10% DMSO (Sigma).

The medium was decanted and each well was washed twice with 1 × PBS.

The plate was decanted and blocked with RPMI medium containing 10% FBS at 37 °C for 2 h.

After three days of culture, PBS was decanted into special culture vials containing medium for aerobic (BD BACTEC Plus Aerobic/F, BD and Company, Sparks, MD, USA) or anaerobic (BD BACTEC Plus+ Anaerobic/F, BD and Company) bacteria, respectively.

After five days of culture, PBS was decanted into a special glass tube containing medium for fungi (Sabouraud 2 agar (SAB2-T), BioMerieux, Marcy l'Etoile, France).

On day 3, 100  μl of the culture supernatant was decanted and replaced by an equal volume of fresh medium supplemented with 20 ng ml−1 IL-7 and 200 pg ml−1 IL-12.

The supernatant was decanted in the glove box, and 25 mL of complex medium (including 5 mM IPTG for E. coli cells) was added.