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The medium was continuously stirred and maintained at 25.0 or 31.0 ± 0.5 °C using a water bath.
The perfusion reactor was connected via tubing to a medium reservoir bottle from which medium was continuously pumped through the reactor (Fig. 1A,B).
Then, the release medium was continuously agitated with stirring speed 100 rpm at 37°C.
The dissolved oxygen concentration in the medium was continuously monitored using a DO probe (Mettler Toledo).
The growth medium was continuously renewed until the amount of 10 g L−1 wet mass was achieved to be added to the medium.
The activity of RDPE in medium was continuously increased and reached the maximum of 65 U/ml with a high productivity of 0.9 U/ml h at 72 h.
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The model can be applied by means of conventional equations of state and is primarily useful for studies of physical processes in which the (hypothetical) fluid medium is continuously varied.
In this operation, the material removal occurs from any electrically conductive material by the initiation of rapid and repetitive spark discharges between the gap of the work and tool electrode connected in an electrical circuit, and the liquid dielectric medium is continuously supplied to deliver the eroded particles and to provide the cooling effect.
Sensor chips with cells were then transferred to the Bionas 2500 analyzer in which medium is continuously exchanged in 8-min cycles (4 min exchange of medium and 4 min without flow) during which the parameters were measured.
Ethanol (0.2%) was added to the medium, which was continuously fed to the fermenter.
The medium pH was continuously controlled using a pH electrode (N 6000).
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