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Good consistence between FW H/V with SW H/V of the corresponding cap-layered medium was confirmed.
Secondary thickening of the wall of cells cultured in the induction medium was confirmed by microscopic examination and correlated with an increase in the lignin content and changes in the polysaccharide composition of the cell wall.
The induction of elpS in poor medium was confirmed by a β-galactosidase assay performed on a strain carrying the lacZ reporter gene from E. coli K12 under the control of the elpS promoter (Fig. 3E).
The regeneration of plantlets via indirect shoot organogenesis on A10 medium was confirmed using histological techniques.
Expression and secretion of B4GAT1dTM protein into the culture medium was confirmed by immunoblotting with anti-Myc antibody 4A6 (Millipore).
The final FFA concentration in the medium was confirmed with an enzymatic colorimetric assay (NEFA C, Wako).
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If a frequency-dependent effect of the extracellular medium is confirmed by future studies, Eqs.
For each experiment, the cells shed into the medium were confirmed to be morphologically apoptotic with condensed chromatin.
The inoculated tubes were examined up to 8 weeks and colonies grown on LJ medium were confirmed by ZN staining.
Colonies isolated on selective medium were confirmed as E. coli O157 H7 following the instructions of latex agglutination kit (BD Difco™E. Coli Antisera kit from Becton, Dickinson and Company, Cockeysville, MD).
Sal-resistant mutants were identified by the ability to form colonies on this medium, were confirmed by re-streaking on the same medium, and were demonstrated to contain plasmid-linked Sal-resistant mutations by preparing plasmid DNA, transforming E. coli D21f2tolC with plasmid DNA, and plating transformants on the same medium.
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