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While the medium conditioned by Kupffer cells inhibited hepatocyte DNA synthesis, which was augmented when the medium was conditioned in the presence LPS, ALR's presence in the medium during conditioning by Kupffer cells reversed the inhibitory effect [ 22].
Medium was conditioned for 22 26 h, pooled, filter sterilized and supplemented with an additional 4 ng mL−1 FGF2 and stored at −20°C.
In addition, we monitored 72 hour survival of FHF-induced rats as a function of MSC mass from which medium was conditioned (Fig. 2B).
In certain wells, the medium was conditioned by MØ polarized in the presence of 50 µM or 100 µM Nor-NOHA, a specific arginase inhibitor or in the presence of 1 µM pioglitazone or 1 µM GW9662.
Fresh medium was conditioned for 24 h before relocalization analysis of hnRNP A1 by immunocytochemistry.
The same medium was conditioned by exposure for two days to logarithmically growing mouse 4T1 mammary carcinoma cells and subsequently filtered through 0.2µ pore sized filters.
Similar(54)
BMSC monocultures were supplemented either with fresh chondrogenic medium or with chondrogenic medium that was conditioned for three days by OAB explants.
Specifically, the culture medium contained 50% regular and 50% conditioned medium that was conditioned for 48 h and sterile filtered.
Briefly, 5 × 10 cells were seeded in a 60 mm dish, and culture medium that was conditioned for 24 h was used for the ELISA assay.
This medium was used after being conditioned on monolayers of cortical glia for 24 hours.
Conditioned medium: LADMAC conditioned medium was generated by culturing LADMAC cells in medium as described above until confluence.
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