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The coarse sand in the upper portion of the porous medium was cleaned rapidly by the surfactant flushing.
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The culture medium containing lentivirus particles was cleaned by centrifugation to get rid of the cell debris at 12 000 × g for 5 min and was used to infect the target cells.
The culture medium containing lentivirus particles was cleaned by centrifugation to get rid of the cell debris at 12 000 × g for 5 min, and used for the target cells infection.
RNA was cleaned with RNA Clean-up kit (Macherey-Nagel).
The intestine was cleaned further by gently moving it, with the mucosal layer facing downwards, in a Petri-dish with approximately 1 ml medium.
Immediately after collection, the spent medium samples were cleaned of cell debris by a brief centrifugation step.
24 hours later, the medium was removed, and wells were cleaned with DPBS (Sigma).
If the medium solid is magnetite, it can very easily be cleaned from slimes using its magnetic property.
First, single ciliates were cleaned as described (Fig. 1, steps 1 3) and then starved in Woods Hole MBL medium (Guillard & Lorenzen, 1972) for some days.
Pens were cleaned weekly.
Data were cleaned.
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