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The release behavior of the phenolic compounds from the films in an aqueous medium was assayed finding significant differences (p < 0.05) between release rates of both extracts.
Conditioned medium was assayed for radioimmunoassayable Sm-C.
The recombinant plasmids were transfected into mouse neuroblastoma cells, and the cell medium was assayed for the human growth hormone.
MMP released from SK-Hep1 cells into the medium was assayed by gelatin zymography (7.5% zymogram gelatin gels) (Hung et al. 2009).
H2O2 in the medium was assayed by Amplex Red per the manufacturer's instructions.
To gain insight into the intracellular location of Obelix, a myc-tagged version was transfected into COS-1 cells, and the presence of Obelix protein in cell lysates and medium was assayed by Western blotting.
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ATPS with different compositions of polyethylene glycol 4000 PEG40000) and 10-fold concentrated saline medium were assayed during extractive fermentation processes.
Putative transgenic RO papaya plants, regenerated on kanamycin-containing medium, were assayed for GUS and PRV coat protein expression, for the presence of NPTII and PRV cp genes [with the polymerase chain reaction (PCR) and genomic blot hybridization analysis], and for PRV cp gene transcripts by Northern analysis.
Various G-CSF concentrations with conditioned medium were assayed by using Ready-Set-Go ELISA kit (R&D systems) as described by the manufacturer.
Forty-eight hrs later, cells were treated with different concentration of DTT and aliquots of the conditioned medium were assayed for Gluc activity either 4 hrs later (For the DTT and thapsigargin dose-response curve) or at different time points (for real-time monitoring of ER-stress) as above.
IL-6, TNF-alpha and IL-1beta in the medium were assayed using a multiplex biometric ELISA-based immunoassay, containing dyed microspheres conjugated with a monoclonal antibody specific for a target protein and a procedures already used in our previous article [37].
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