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Treatment outcomes were ascertained for 225 persons.
To confirm the lethal effect of OC for promastigotes, treated and untreated parasites (from the growth kinetics study) were washed and resuspended in fresh medium and their viability was ascertained microscopically after 96 h of incubation.
Specificity was ascertained by degrading the drug sample in alkaline medium.
T cell activation in response to the CII peptides was ascertained by detection of Th1-specific IFN-γ levels in the culture medium following restimulation.
Mortality was ascertained through verbal autopsies.
Mortality was ascertained in 2007.
Muscle mass was ascertained from DXA scanning.
The purity was ascertained by SDS-PAGE.
Glucocorticoid exposure was ascertained from dispensary data.
Blood pressure was ascertained under standardized conditions.
Ovulation was ascertained by ultrasound examination.
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