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No contrast medium was applied during MRI.
The cells were left for 1 h at 37 °C to allow attachment after which 1 mL of pre-warmed culture medium was applied on each well.
A two-step procedure, employing acidic anhydrides (hexanoic or butyric anhydride) as the acylation agent and methanesulfonic acid both as the catalyst and the reaction medium, was applied.
In LIG formulations, where a more fluid ink medium was applied, deterioration starts earlier, at the second day of ageing, with a ΔΤ of 8.1 °C, with ΔΤ reaching up to 12.6 °C at longer times.
Clarified culture medium was applied to a strong anion-exchange (Q) column and PAC was purified by non-retentive separation, where most contaminant proteins bound to the chromatographic media with PAC being collected as the major component in the flow-through fraction.
When unprocessed p87-Sema3E-conditioned medium was applied, no enhancement in cell migration was observed (data not shown).
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Then, the extraction medium is applied into the test system.
The principle of effective stress for porous medium is applied to analyzes the fluid-structure coupling in grouting.
The thermal-controlled nanostructured reaction medium is applied to the ene reaction, [4+2] cycloaddition and sulfide oxidation.
Five differing volume system ratios of the culture cells' extracellular medium were applied for 24 and 48 h of incubation in this study.
Thus the reactor overall behaves adiabatically, while the catalyst bed is nearly isothermal, while no active heat exchange with a second medium is applied.
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