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Medium was analysed for IL-6, IL-8, IL-10 and TNF-α by ELISA (R&D Systems).
Insulin concentration in the medium was analysed using a radioimmunoassay kit specific for rat/mouse insulin (Linco, St Charles, MO, USA).
The role played by iron and its relationship with the aTf used to supplement the culture medium was analysed by iron supplementation experiments and evaluating the DNA content in CTL and aTf-treated N20.1 cells.
When 20 μg of protein from conditioned medium was analysed, the separation between proMMP9 and MMP9 bands was lost and a large band of lysis was also seen at a size corresponding to MMP2.
Pro-MMP-2 and pro-MMP-9 expression in conditioned medium was analysed using 10% SDS-gelatin (1 mg ml−1 final concentration) zymography under nonreducing conditions as described previously (Ahmed et al, 2002c).
The insulin content of the incubation medium was analysed using the high-range HTRF insulin kit (62INSPEC, Cisbio Bioassays, Codolet, France) and fluorescence resonance energy transfer was measured on an Envision plate reader (PerkinElmer, Cambridge, UK).
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Hydrolysis experiments carried out using thin glass tanks filled with glass beads to construct a hypothetical and inert, homogeneous porous medium were analysed using a 2D numerical model.
The corrosion inhibition properties of a new class of oxadiazole derivatives, namely 3,5-bis n-pyridyl -1,2,4-oxadiazoles (n-DPOX) for C3,5-bis n-pyridyl -1,2,4-oxadiazoles medium were analysed by electrochemical impedance spectroscopy (EIS).
The influences of the current density, pH, additives, and concentration medium were analysed.
Cytokine levels in the cell culture medium were analysed with a SECTOR Imager 2400 reader (MesoScale Discovery, Gaithersburg, Maryland, USA).
viciae 3841and the prsD (A895), toaD (A913), tobD (A896), virB6 (autA), A1010(autB0), A1011(and11) autCA1012(A1012) mutants grown in Y-mannitol medium were analysed by SDS-PAGE.
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medium was identified
medium was calculated
medium was quantified
middle was analysed
medium was assessed
medium was investigated
medium was explored
medium was characterized
medium was evaluated
media was analysed
medium were analysed
medium was refreshed
medium was provided
medium was renewed
medium was selected
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