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The medium was amended with acetate as an electron donor and carbon source, with an initial concentration of 650 mgCOD L−1 (600 mg/L as acetate).
Recently, Manavalan et al. (2013) described significantly increased (1.5 U ml−1) for Lcc production by Ganoderma lucidum when the culture medium was amended with 0.4 mM CuSO4.
To assess the effects of co-substrate addition of PHA synthesis, the medium was amended with varying concentrations of 4HB, 5HV, and 6HHx monomers.
The culture medium was amended to cover the tissue at the next day.
This base medium was amended using various vitamin or nutrient substrates (Tables 1– 3).
RM medium was amended separately by adding 5 mM each of 4-hydroxybenzaldehyde, syringaldehyde, or vanillin for testing the inhibitor tolerance.
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The basal medium (MMN) was amended with CMC, 1.0% (w/v) (Caldwell et al. 2000).
When required, kanamycin (100 μg/mL) was amended to the medium.
W27-amended core was amended with 15NO3-.
Water was amended with NaCl to produce a control runoff (no added salt), a medium (500 mg/l) and a high (1500 mg/l) salinity runoff.
That communiqué, too, was amended.
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