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Initial medium volume was 3 L, and air aeration rate was 1.33 vvm.
All the components are autoclaved separately and mixed subsequently so that the final medium volume was 50 ml in 250 ml Erlenmeyer flask.
The next day, the total medium volume was adjusted to 700 ul and virus particles were diluted in 50 ul of DMEM+ prior to well addition.
One-third of medium volume was exchanged every 5th day.
Subsequently, half of the culture medium volume was refreshed with new SFDM every other day.
Then, half of the medium volume was replaced every 48 72 h.
Similar(50)
In fact, the highest titre of each dog during this study (range 101.7 to 102.8 EID50/200 μl, Table 3) was apparently lower than those of horses inoculated with EIV in the previous study (range 103.5 to 104.3 EID50/200 μl) [ 11], although the sampling conditions (swab size and medium volume) were admittedly different between dogs and horses.
Also smaller contrast medium volumes are needed for administration of an identical iodine dose.
Because epididymides size varied among species, we used between 0.25 ml and 3 ml of mT-H medium and volume was recorded for the calculation of total sperm numbers.
Culture medium (half of volume) was replenished with fresh medium containing mIL-2 every 3 days.
It should be noted that 25 ml was almost the lowest volume limit of BMMY medium; a lower volume was not viable for cell growth, enzyme expression, and triglyceride hydrolysis because no free water remained during the cultivation process.
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