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For a given production rate, the concentration in the downstream MPS of the production location will be maximized by increased inter-MPS flowrate and reduced medium volume of the downstream MPS.
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The cultures were grown in cotton-stoppered baffled shake-flasks with starting medium volume of 10% of the flask volume.
Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
In our study, the optimum conditions (seeded cell number, incubated time period, 12-azidododecyl lactoside concentration and medium volume) for the production of Gb3 analogue were investigated.
By using single-particle electron microscopy and three-dimensional reconstruction, we obtained a medium-resolution volume of the human GINS complex, which shows a horseshoe shape.
By using single-particle electron microscopy and three-dimensional reconstruction, we have obtained a medium-resolution volume of the human GINS complex showing its horseshoe shape.
All the aerobic batch cultures were cultivated in baffled shake flasks with a medium volume equivalent to 10%% of the volume of the baffled shake flask to maintain adequate aeration.
Using the phase mask allows effective use of the medium volume and its dynamic range and improves reconstruction performances of stored holograms.
The EBCT and organic loading rates were recorded on the basis of the medium volume.
It was added to each well of 96 wells plate, a volume of 15 μl Alamar Blue solution, which represent 10% of the medium volume in each well.
No outgrowth of cells was observed when cultivating medium volumes devoid of cells aspirated from the diluted cell suspensions.
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