Exact(3)
Mr. Williams is dismissive of those who say making Medium viable is not merely difficult, but flat-out impossible.
Considerations for setting up microscopy analysis included the fixing protocol, choice of growth medium, viable cell counting, cross-talk between fluorophores, as well as the method of 3D visualization.
After treatment, cells were washed with PBS and incubated with fresh drug-free medium; viable cells number was estimated by means of a Coulter Counter (Beckman Coulter Corp., Hialeath, FL, USA) at different time intervals after drug-washout.
Similar(57)
Yes, we should tell them about the long-view government spending that paid the initial salaries, and the entrepreneurs who figured out a way to make the new medium commercially viable.
"But has it been too long since the medium was viable in this area?" "This is a perfect moment to do it," she added, "but I think they get one shot to do it".
Samples were periodically withdrawn and plated on rich medium for viable cell counts (Methods).
The splenocytes were washed twice and resuspended in the culture medium, and viable mononuclear cell numbers were determined by trypan blue exclusion [32].
The remaining cells were washed twice with culture medium and viable mononuclear cell number was determined by counting Trypan blue unstained cells in a hemocytometer.
Upon being added to medium containing viable cells, MTS is reduced to a water-soluble formazan salt.
In some cases, we assessed MMP activity in conditioned medium from viable free-floating spheroid cells after MEC co-culture.
The digested tumour suspensions were washed twice in RPMI 1640 medium, and viable cells were isolated on a two-layer Ficoll gradient.
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