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In these experiments, ammonia production was performed in M9-YE medium using yeast extract as the sole source of carbon and nitrogen.
Fig. 1 Profiles of glucose concentrations, DCW, total lipids and ARA yield in a 3.6-L bioreactor by M. alpina cultured in the medium using yeast extract as the sole nitrogen source.
To address this issue, the experiment was repeated in glucose-based medium using yeast strains bearing a chromosomally-integrated allele (iSWI6-GFP) under the control of the endogenous SWI6 promoter.
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Total RNA was extracted from isolates grown in YPD medium using the Yeast RNAiso Reagent Kit (TaKaRa, Tokyo, Japan) and reverse transcribed to cDNA using the PrimeScript RT Reagent Kit (TaKaRa, Tokyo, Japan) according to the instructions of the manufacturer.
Dextrose, sucrose, and raffinose assimilation tests were performed in triplicate by using yeast nitrogen base medium.
Shadomy modified medium was used (Yeast Nitrogen Base, asparagine and glucose) with phosphate buffer to pH 7 and containing chloramphenicol to avoid bacterial contamination.
Fungus was isolated as described earlier (Kaur et al. 2014) using yeast extract glucose agar (YGA) medium.
In this study a natural culture medium that mimics the synthetic yeast peptone glucose medium used for yeast fermentations was designed to screen and select yeasts capable of producing high levels of diacetyl and acetaldehyde.
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The medium used for yeast strain maintenance was YPD containing 1% (w/v) yeast extract, 2% (w/v) peptone, and 2% (w/v) glucose.
The medium used was yeast extract peptone dextrose (YPD) supplemented with Geneticin (200 μg/mL) (GIBCO, Rhenium Modi'in, Israel) or nourseothricin (Nat) (100 μg/mL) (Werner Bioagents, Jena, Germany) or hygromycin-B (300 μg/mL) when required.
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