Sentence examples for medium using sterilized from inspiring English sources

The spores were washed from the medium using sterilized distilled water.

The first subculture method used was as follows: (1) cells were gently rinsed away from the monolayer with culture medium using a sterilized pipette or by tapping the bottom of the flask to release attached cells; (2) the cell suspension was transferred into a new 25 cm2 flask at a ratio of 1∶2 and were supplied with an equal volume of fresh medium.

For these experiments, the yeast strains were allowed to partially thaw at room temperature and a small sample of each strain was transferred to 150-mm × 15-mm Petri dishes containing solid YPD medium using a sterilized 96-pronged metal replica plater.

The growth of the freshwater green algae Raphidocelis subcapitata (Culture Collection of Algae at the University of Göttingen) was tested based on OECD guideline 201 [31] using sterilized OECD medium with tenfold increased iron content.

Sorbitol and sodium chloride were added to VM agar medium prior to autoclaving, while all other chemicals were added to cooled autoclaved VM agar medium using filter-sterilized concentrated stock solutions.

The incubated medium was harvested by filtration with sterile cheese cloth, washed using sterilized Milli-Q and it was grinded in liquid nitrogen.

Confluent HEC-1 cells were scraped using sterilized tips, and incubated in culture medium for 24 h.

After 1 week Matrigel implants were harvested from the animals, minced in small pieces using sterilized razor blades and mechanically dissociated with a micropipette in 400 µl of tissue culture medium [20].

Bioreactor chambers were constructed using sterilized thin-walled polystyrene boxes housed in calibrated temperature units.

All sample transfers were conducted using sterilized implements and containers.

Dental examination was conducted using sterilized dental mirrors.