After a 30 min incubation period at room temperature, the samples were evaluated spectrophotometrically at 548 nm and OD values – in comparison with a standard curve was determined in culture medium by using serial dilutions of sodium nitrite – represented total stable metabolites of NO.
Efflux mechanisms were investigated by broth dilution in Mueller-Hinton medium containing 5% sheep blood using serial dilution of ciprofloxacin in the presence or in the absence of 10 mg/L reserpine (Sigma-Aldrich, St . Louis MO).
FIX protein levels in plasma and in conditioned medium were evaluated by ELISA (Affinity Biologicals™ Inc., Canada) using serial dilutions of a PNP as reference curve.
The amount of nitrite present in the samples was calculated by means of a standard curve generated using serial dilutions of NaNO2 in fresh culture medium.
Bacterial quantifications for homogenized liquid were made using serial 10 fold dilutions in the sterile salt solution (NaCl 0.85%), followed by plating on an agar medium.
Using serial dilution and enrichment, mesothelial cells (Hospicells) interacting specifically with the ovarian cancer aggregates were isolated and grown at 37°C in 5% CO2/95% air in RPMI medium (Mediatech Inc).
Tumour growth was monitored using serial BLI.
US spelling uses serial comma.
Thereafter, serial dilution (10−1 to 10−5) were made suing sterile PBS and bacterial strains were plated on Petri-dishes solid LB-agar mediusinging an L-spreader.
