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Osteocalcin was measured from cell culture medium using Mouse Osteocalcin EIA Kit (Biomedical Technologies Inc., MA) according to the manufacturer's protocol.
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TNF-α was measured in cell lysate and undiluted medium using the Mouse TNF-α Immunoassay Kit from R&D Systems (Minneapolis, MN).
The cells were re-plated in a 10 cm culture dish with irradiated MEF on 0.1 % gelatin using mouse ESC medium.
Also on Monday, Berkeley officials gave Bustamante and reporters a tour of a lab in the mining building that is developing a synthetic material that would be a better medium on which to grow stem cells than the current medium, which uses mouse cells and is vulnerable to contamination.
mPBMCs were isolated from C57BL/6J mice using mouse lymphocyte separation medium (IBL, Japan), and 0.5 × 10 cells were cultured in RPMI.
In the case of EB formation using mouse testicular or ovarian cell-conditioned medium (see below), the EBs were cultured in ES medium for 24 hr, and then in a conditioned medium.
The efficiency of developed microarrays was demonstrated using mouse IgG and goat anti-mouse IgG as a model affinity pair.
The second strategy is using mouse model.
The standard curve was mouse apoE purified from mouse astrocytes conditioned medium using a polyclonal antibody (Calbiochem).
This study involved using mice.
This study utilized experiments using mice.
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