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The titer of FAEE in YL15 strain reached to 813 mg L−1 in minimum medium using glycerol as sole carbon source under optimized fermentation conditions.
This was done by comparing the growth rates of T-DNA tagged mutant colonies on solid medium using glycerol as the sole carbon source.
Cells were grown anaerobically in a minimal medium using glycerol as sole carbon source and nitrate as terminal electron acceptor.
SMP2 medium, a synthetic minimal medium modified from P2 medium using glycerol and sodium pyruvate as the basic carbon sources, was adopted to grow C. acetobutylicum cells for the analysis of the "glucose effect" for bukII gene.
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After washing in PBS, the slides were mounted using glycerol mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) and 1,4-diazabicyclo 2,2,2 octane (DABCO) (Abcam, Cambridge, UK).
After rinsing, the brain sections were mounted on gelatin-coated slides and covered with a cover glass, using glycerol buffer as a mounting medium.
Stock cultures were maintained on Reinforced Clostridial Medium (RCM, Oxoid Deutschland GmbH, Wesel, Germany) and preserved using glycerol 20%% (v/ v) at −80 °C.
To investigate whether ZS1 isolate produced rhamnolipids using glycerol as sole carbon source, we analyzed the growth of ZS1 in MS medium supplemented with 1, 2, and 4% glycerol.
In the view of using glycerol as a carbon source in industrial applications, it is a promising result that certain S. cerevisiae strains are able to grow in synthetic glycerol medium without complex supplements.
We mixed a culture of strain HdN1 with a culture of luminous bacteria (isolated from herring using glycerol-peptone medium; Farmer and Hickman-Brenner, 2006) as sensitive O2 indicators (Chance et al., 1978); both cultures had been adapted to brackish water (180 mM NaCl and 20 mM MgSO4) medium.
In addition, the RSM optimization of the half-cell performance for glycerol oxidation in alkaline medium using the PdAu/VGCNF catalyst has never been studied.
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