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The final strain produced up to 8.31 g/L NeuAc in minimal medium using glucose as sole carbon source.
The effect of immobilization of S. stipitis in a calcium alginate matrix was first investigated in rich medium using glucose or xylose as sole substrate in shake flasks.
We then evaluated cadaverine production by Jm-cadA, Jm-blc-Tfu and Jm-cadA-blc-Tfu grown in R/2 medium using glucose as the sole carbon source.
Transformants were grown in YNB medium using glucose as the carbon source.
Fed-batch cultures were performed in minimal medium, using glucose as the carbon source, at 37°C.
Although the deletion mutant for GLK1 whose product is also known to phosphorylate glucose at the C6 position was not investigated, we predict that its deletion would yield results comparable to those of HXK1, as both GLK1 and HXK1 are repressed when grown on a fermentable medium using glucose as a carbon source [ 54].
Similar(54)
This phenomenon was studied in fed-batch culture under optimal operating conditions, with a mineral medium and using glucose as carbon source.
The fermentation was carried out at 1 4 moisture content and initial medium pH 5.0 using glucose as a co-substrate.
The concentration of glucose in cultivation medium was measured using glucose oxidase method with a glucose assay kit (Sigma-Aldrich Corporation, St .Louis, Missouri, USA).
Glucose or lactate were measured in cell supernatants 3 hours after changing medium using a Glucose Assay Kit (Sigma Aldrich) or a Lactate Colorimetric Assay Kit (Abcam).
Also, wouldn't the induction of both the human deaminases and I-SceI be better if, instead of using glucose medium at all, cells were grown in the non-inducing/non-repressing mediums of either raffinose or 3% glycerol 3% lactate, followed by the addition of galactose?
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