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Indeed, the medium used during exposure prevents electrostatic interactions between bacterial cells and nanoparticles, leading to false-negative responses.
The carbon sources in the feed medium were prepared based on the C-molar concentration of 20 g/L glucose (0.666 Cmol/L) as in the medium used during batch cultivation.
Besides, we have noticed a progressive dehydration of the medium used during the course of the prolonged incubation needed for the demonstration of NB, a process that may take upwards to 2 months, and this gradual change in fluid conditions by itself can be shown to lead to nanoparticle precipitation if not properly controlled (data not shown).
The volume of contrast medium used during urgent coronary intervention tends to be relatively higher than in elective procedures.
The medium used during reprogramming was supplemented with L-ascorbic acid, which was removed after the initial selection of clones.
The carbon medium used during the start-up contained the following: acetic acid, propanol, butanol and methanol (see Materials and methods).
Similar(52)
Due to nanoparticle aggregation in serum-containing medium, serum-free medium was used during 4-h exposure.
Thus, we tested the efficacy of WAY on IhERG after diluting the drug: (1) in SES, normally used for our patch-clamp experiments; (2) in the culture medium used for growing glioma cells during secretion experiments (CM); (3) in CM medium preincubated for 24 h in the same dishes containing the cells (CM 24 h).
Strains were grown overnight (37°C, 200 rpm) and separately diluted 1/100 in fresh medium, all culturing prior to inoculating the microhabitat was done in the same medium as used during the experiment.
B-25, also called Mitchell, U.S. medium bomber used during World War II.
B-26, also called Marauder, U.S. medium bomber used during World War II.
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