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Osteogenic differentiation capacity of ADSCs can still vary according to the culture medium used, as well as by adding factors that can alter signaling pathways and enhance bone differentiation.
The dynamic approach has been applied to suppositories from which paracetamol has been extracted in this way into an aqueous phase and hydrolysed prior to reaction with o-cresol in the alkaline medium used as extractant.
The SDF-1α released from PLGA/SDF-1α and PLGA/BSA-SDF-1α particles retained its chemotactic activity, and enhanced the number of migrated mesenchymal stem cells (MSCs) by 38%and54%4%, respectively, compared to basal medium used as the control.
The cell culture medium used as a negative control was not cytotoxic.
HUVEC cells were grown to confluence and then incubated with serum free RPMI medium (used as control), 200 and 500 ng of recombinant VEGF165.
Using the C. albicans database, 976, 18, and 0 proteins were identified from the C. albicans monoculture, the macrophage monoculture, and the complete culture medium, used as background, respectively (Additional files 1, 5).
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The E. coli strains and mediums used as the expression host and the gene engineering host were described previously (Sakaguchi et al. 2015).
YES or YEA medium was used as complete medium, and EMM2 medium, containing nutritional supplements when necessary, was used as minimal medium.
Hank's medium (H15-010), used as starvation medium, was obtained by PAA.
EMM2-N (EMM2 lacking a nitrogen source) medium and ME medium were used as sporulation media.
Freshly prepared Muller Hinton Broth (MHB) medium was used as a base medium for the assay.
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