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The experiments were performed in liquid medium under stationary conditions at 28°C in the dark.
The PHB production was followed in 250 mL Erlenmeyer flask containing 50 mL sugar industry waste water as production medium under stationary condition of growth.
The fermentation was carried out in 250 mL Erlenmeyer flask containing 50 mL sugar industry waste water as production medium, under stationary as well as shaking condition of growth.
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The same study also supports our results concerning the pasteurized milk: usually, only low to medium cereulide levels are produced under stationary conditions at room temperature [ 30, 37].
Optimal production was obtained when P. glabrum was grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days.
Inoculum was prepared by transferring a loopful of fungal culture from slant to 100 mL sterilized cornmeal medium and incubated at 20°C for 7 days under stationary conditions.
Subsequently, the scaffold was cultured under stationary conditions in the incubator and the culture medium was refreshed every 24 h.
The production culture was initiated by transferring 3 mL seed culture into each of fifty 500 mL culture bottles (As one, Osaka, Japan) containing production medium, and the fermentation was carried out at 27 °C for 14 days under stationary conditions.
Bacteria were grown under four different conditions: log phase in LB medium, stationary phase in LB medium, MgM dilution (AMM1), and MgM medium shock (AMM2) as described previously [ 24].
The optimization for maximum PHB production by Bacillus subtilis NG220 was carried out under stationary conditions of growth using sugar industry waste water as production medium.
Clostridium thermocellum DSM 1313 cells prepared under different conditions (washed and resuspended in distilled water, actively growing in medium, stationary phase in medium, autoclave killed, grown with and without resazurin) were incubated in 150 mg/L methyl viologen (Sigma Aldrich) to determine the origin of the reductant being used to reduce methyl viologen by growing cultures.
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