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The strains were cultured in N5G medium under standard (open symbols) and optimized (filled symbols) culture conditions.
A preliminary study revealed that CA production was markedly increased when 1% methanol and 10% SVH were added to basal medium under standard fermentation conditions.
For all experiments, unless otherwise noted, Podospora anserina wild-type strain s [13] was used, grown on standard cornmeal medium under standard conditions [14].
All lines were maintained by full-sib mating for 10 generations on cornmeal-molasses-agar medium under standard conditions of 25±1°C, 70% humidity and a 12-h light∶ 12-h dark cycle.
HeLa cells were cultured in DMEM medium under standard conditions.
SCC13, SCC25, SJG cell lines, and normal keratinocytes were cultured in FAD medium under standard conditions.
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Strains were analysed for growth in axenic medium both under standard conditions as well as under osmotic stress conditions by addition of 115 mM sorbitol or 30 mM NaCl.
Cells were cultured in complete medium (CM) under standard conditions at 37°C and in humidified 5% CO2.
Most yeast genes are non-essential, i.e. dispensable, in rich medium or under standard laboratory conditions (>80%, ref. [ 10]).
Adherent cells (HEK293, SAOS-2, MEF, B16F10) were grown in Dulbecco's modified Eagle medium (DMEM) under standard conditions (Sassi et al, 2010).
After infection, cells were pelleted at 300× g, washed three times with 1 ml of culture medium, resuspended in 1 ml of culture medium, and grown under standard conditions.
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medium under various
medium under phototrophic
medium under different
medium under intensive
medium under constant
medium under shake-flask
medium under hypoxic
medium under filtered
medium under certain
medium under recommended
medium under stationary
medium under mechanical
medium under suboptimal
medium under paraffin
medium under pH
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