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The influence of normal and mutant PrP forms on intracellular LIP was evaluated in M17 and transfected cell lines cultured in complete medium under normal culture conditions.
Then, the drug/medium was removed and cells were allowed to incubate in fresh medium under normal conditions for 7 days.
Importantly, no SEL1L immunoreactive bands are found in the MCF10A culture medium under normal and ER-stressed conditions, suggesting that, at least in cells of breast epithelial origin, secretion of the two soluble SEL1L forms is associated with the tumorigenic phenotype.
In SD medium, under normal growth condition, PL fraction contained slightly less EPA than total lipid fraction.
Retinal PCs were cultured in medium under normal, high glucose or osmolarity control for 5 days as detailed and previously used by us and others.
Conditioned medium from PCs under high glucose conditions inhibited migration of ECs compared with PC conditioned medium under normal glucose or control.
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Additionally, proliferation and migration of ECs and SMCs differed between conditioned medium collected under normal gravity and under simulated microgravity.
The SMC-conditioned medium cultured under normal gravity (SMC CM + 1 g) and simulated microgravity (SMC CM + MG) condition was added to study EC migration capacity.
For each strain, at least four different clones were sequenced, each clone deriving from a single colony grown on solid medium and under normal growth conditions.
As shown in Figure 6(C) (upper panel, lanes 3 and 4), HeLa cells transfected with TMPRSS6 Y141C) failed to release cdTMPRSS6 into the medium, both under normal and reducing conditions.
Briefly, cells were seeded in 96-well plates at 1×103 cells per well in 100 µl of culture medium and grown under normal conditions at 37°C in humidified atmosphere.
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medium under phototrophic
medium under sterile
medium under anaerobic
medium under non-selective
medium under physiological
medium under constant
medium under initial
medium under high-temperature
medium under hypoxic
medium under microaerophilic
medium under recommended
medium under stationary
medium under static
medium under various
medium under intensive
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